Abstract: In this work, a molecularly imprinted polymer (MIP) of morphine (MO) was prepared through thermal radical copolymerization of methacrylic acid (MAA) and ethylene glycol dimethacrylate (EDMA) in the presence of MO templates, and a molecularly imprinted sorbent assay (MIA) based on a colorimetric reporter was developed to determine the adsorption isotherm of MO-MIP binding. In practice, the MO-bound MIP was brought into contact with an aqueous mixture of Fe3+ and [Fe(CN)6](3-) so that the 3-phenolic group of MO was oxidized and Fe3+ was reduced to Fe2+. As a result, the MO-bound MIP was stained with Prussian blue (PB), which was attributed to the instant co-precipitation of Fe2+ and [Fe(CN)(6)](3-) (K,P = 10(-40)). Accordingly, MO- MIP binding of the blue dye could be detected by visible spectroscopy. In addition, such staining could successfully distinguish MO from codeine. Upon data analyses, a two-site binding isotherm with two dissociation constants of 6.00 x 10(- 5) and 1.03 x 10(-3) M was found for MO-MIP binding. MIAs for non-MIP were also performed. In addition, the results of flow- system characterizations and the particle size effect are also described in this paper.(C) 2003 Elsevier B.V. All rights reserved

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