Abstract: Determination of uric acid in human serum and urine is useful to provide treatment guidelines to hyperuricemic patients. An electrochemical sensor was developed for selective and quantitative recognition of uric acid by using a preanodised sol-gel coated graphite electrode with a molecularly imprinted polymer brush of poly(melamine-co-chloranil) grafted to its exterior surface. During a preconcentration step at (+á2.0áV versus saturated calomel electrode), the encapsulated analyte recapture involved hydrophobically induced hydrogen-bondings in outwardly exposed MIP cavities in aqueous environment (pH 7.0), instantly oxidised as dications, and then cathodically stripped off as corresponding lactam responding differential pulse, cathodic stripping voltammetric signal. The uric acid was selectively detected without any cross reactivity in the windows of 14.56-177.42áÁg mL-á1 (aqueous medium), 4.78-106.96áÁg mL-á1 (blood serum), and 7.81-148.42áÁg mL-á1 (urine) indicating detection limits in the range of 3.71-4.10áÁg mL-á1 (3σ, RSDá=á1.9%)
Template and target information: uric acid
Author keywords: Uric acid sensor, Molecularly imprinted polymer brush, sol-gel, Graphite electrode, differential pulse, Cathodic stripping voltammetry

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