Abstract: Molecularly imprinted polymers (MIPs) for recognition of punicalagin were prepared by precipitation polymerization using punicalagin as template, acrylamide (AM) as the functional monomer, ethylene dimethacrylate (EDMA) as the cross-linker, azoisobutyronitrile (AIBN) as the initiator and acetonitrile as the solvent. The MIPs prepared formed spherical beads with nanometer sizes. The results of equilibrium adsorption experiments showed that the MIPs had a high affinity to the template in comparison with the blank polymers (BPs), and the max adsorption of MIPs was 32.6 μmol/g under the studied concentration; Scatchard analysis showed that two kinds of binding sites were formed in the MIPs and one kind of binding site in the Bps, the apparent maximum binding capacity was 243 μmol/g, whilst for the Bps, the apparent maximum binding capacity were 25.3 μmol/g; the selective binding experiment showed that the MIPs had higher selectivity and affinity
Template and target information: punicalagin
Author keywords: microsphere, molecular imprinting, precipitation polymerization, Punicalagin

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