Abstract: Abstract: Molecular-imprinted membranes of Puerarin were prepared by phase inversion technique with acrylonitrile - acrylic acid copolymer (P (AN-co-AA)). To characterize P (AN-co-AA), ubbelohde viscometer was used to measure its viscosity - molecular weight. P (AN-co-AA) with different molecular weight was used to prepare membranes. The copolymer - dimethyl sulfoxide solution with Puerarin (PU) template was coagulated in water at various temperatures. The increase in P (AN-co-AA) molecular weight and the decrease in coagulation temperature caused an increase in PU recognition property of the resultant membrane. The PU imprinted membrane prepared with P (AN-co-AA) showed good selective ability to PU. The purity of PU increased from 56.51 to 98.41 wt%. Surface and cross-section morphology of the membranes were analyzed by using scanning electron micrograph. High-performance liquid chromatography was used for the quantification of Puerarin in isolated fraction. SCANNING 33: 7 - 12, 2011. © 2011 Wiley Periodicals, Inc
Template and target information: Puerarin
Author keywords: molecular imprinting, membrane, separation, purification, Puerarin

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