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Reference type: Journal
Authors: Jing T, Xia HA, Guan Q, Lu WH, Dai Q, Niu JW, Lim JM, Hao QL, Lee YI, Zhou YK, Mei SR
Article Title: Rapid and selective determination of urinary lysozyme based on magnetic molecularly imprinted polymers extraction followed by chemiluminescence detection.
Publication date: 2011
Journal: Analytica Chimica Acta
Volume: 692
Issue: (1-2)
Page numbers: 73-79.
DOI: 10.1016/j.aca.2011.02.057
Alternative URL: http://www.sciencedirect.com/science/article/B6TF4-529V166-2/2/5b27a6dd7011324b39edf776cb4ee71e

Abstract: A rapid, low cost and selective chemiluminescence method coupled with magnetic molecularly imprinted polymers extraction was developed to detect lysozyme in human urine samples. Compared with traditional solid-phase extraction, this method could achieve selective extraction for the lysozyme, avoid the time consuming elution from a column or centrifugation steps, and then showed great potential in the high-throughput screening of clinical samples. The parameters affecting the performance of extraction and chemiluminescence were investigated. Under optimal conditions, the whole analytical procedure was completed within 12ámin and spiked recovery ranged from 90.1% to 103.7% (R.S.D. <= 6.7%). The limit of quantitation was 5 ng mL-1. Furthermore, the results obtained by the proposed method were linearly correlated to those by commercial lysozyme detection kit (r = 0.9595). Finally, the validated method was used to measure the urinary lysozyme of renal disease patients and healthy controls. The results confirmed the reliability and practicality of the protocol and revealed a good perspective of this method for biological sample analysis
Template and target information: protein, lysozyme
Author keywords: Molecularly imprinted polymers, magnetic separation, chemiluminescence, lysozyme, urine samples

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