Abstract: The method of using one living/controlled radical polymerization(reversible addition-fragmentation chain transfer radical polymerization,RAFT ploymerization) for the sythesis of molecularly imprinted microsphere was developed.Combined with the precipitation polymerization,the MIP microspheres were prepared using acetonitrile as the dispersant,enrofloxacin as the template molecule,methyacrylic acid(MAA) as the functional monomer,ethylene dimethacrylate(EDMA) as the crosslinker,cumyl dithiobenzoate (CDB) as the RAFT agent and azobisisobutyronitrile(AIBN) as the initiation agent.The effects of the reaction conditions including the temperature and monomer concentration on the preparation were studied.The diameter and dispersity of MIP micropheres could be controlled through the ratio of AIBN and CDB.The results of scanning electron microscopic images showed that better particle size distribution of MIP microspheres with a diameter range of 2.0-2.2 μm was obtained.The equilibrium adsorption experiment showed that the binding ability of MIP for the template molecule was superior to that of non-imprinted polymer.The amount of saturation adsorption for enrofloxacin and lomefloxacin were 37.3 and 19.5 μmol·g-1,respectively,indicating that the MIP microspheres could well recognize the template molecule.
Template and target information: enrofloxacin
Author keywords: molecularly imprinted polymeric microsphere, living, controlled radical polymerization, Reversible addition-fragmentation chain transfer polymerization, Enrofloxacin, precipitation polymerization

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