Abstract: A new method was optimized for rapid screening of 17β-estradiol (E2) in water under 10 min. Molecularly imprinted polymer (MIP) particles (325±25 nm) were added in a water sample at pH 5.5 and 20oC to form a suspension. Fluorescence emission from E2 non-specifically bound onto the MIP particles was first quenched by large gold nanoparticles (43±5 nm). The Stern-Volmer plot was linear, with dynamic quenching constants (Ksv) of 2.9×104 M-1. Fluorescence emission from E2 specifically bound inside the MIP particles was next quenched by small nitrite anions that easily penetrated the imprinted cavities. The Stern-Volmer plot 16 became non-linear, with Ksv=2.1x10 2 M-1 and static quenching constant (V) below 1.0 M-1. The difference between these two emission intensities varied as the initial E2 concentration in water, generating a Scatchard calibration curve with R2 >0.97 from 0.1 to 10 ppb.
Template and target information: 17β-estradiol, E2
Author keywords: 17β-estradiol, MIP particles, fluorescence quenching, rapid method, water analysis

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