Abstract: A novel strategy for preparing highly sensitive and easily renewable molecularly imprinted polymer (MIP) sensors was proposed. Using melamine (MA) as the template molecule, MIP particles were synthesized and embedded in a solid paraffin carbon paste to prepare the MIP sensor. MA was indirectly determined from the competition between the reactions of MA and horseradish peroxidase-labeled MA (MA-HRP) with the vacant cavities. The detection signals were amplified because of enzymatic reaction to the H2O2 catalytic oxidation. Sensitivity was markedly improved. Sensor renewal was achieved by a simple mechanical polishing of the sensitive film. The linear range for MA detection was 0.005 - 1 μmol L-1 and the detection limit was 0.7 nmol L-1. The molecularly imprinted solid paraffin carbon paste sensor was used for MA detection in milk samples.
Template and target information: melamine, MA
Author keywords: molecularly imprinted, sensor, Melamine, Horseradish peroxidase, Solid paraffin carbon paste

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