Abstract: Affinity sites for L-aspartic acid (L-Asp) in polyaniline (PAn) were created by two successive processes: first, L-Asp was simply added as template molecules during the polymerization of aniline; second, L-Asp incorporated in PAn backbone was extracted by solvent. PAn with cavities complementary to L-Asp template molecules has been utilized for enantioselective recognition of L- and D-Asp. Enantioselectivity of the imprinted PAn could be attributed to the cavities in the imprinted PAn which was complementary to L-Asp templates both in shape and in positioning of groups. Also in this paper, the structural changes before and after extraction of L-Asp templates were revealed by Fourier-transform infrared (FTIR) spectrum Template and target information:L-aspartic acid, L-Asp Author keywords:polyaniline, aspartic acid, enantioselectivity, molecularly imprinted polymer, IR spectroscopy