Publisher: IACSIT Press
City: Singapore
ISBN: 2010-4618
Volume number: 25
Conference information: Proceedings of the 2011 International Conference on Nanotechnology and Biosensors

Abstract: This study aims at investigating the immobilization of α-amylase on molecularly imprinted polymers. Molecular imprinting is proved to be an effective method that creates three-dimensional binding sites in polymers. Immobilized enzymes have various applications. For optimum use of enzymes and for the ease of isolation and purification, they should be immobilized. In the present study, after the parameters that are effective on enzyme activity and the degree of its change in immobilized state are examined, the optimum conditions for enzyme immobilization are determined. In doing so, 35 ºC and pH=5 are obtained as optimum data for immobilization. Furthermore, the synthesized molecularly imprinted polymer was characterized by scanning electron microscopy (SEM), Brunauer-Emmet-Teller (BET), CHN analysis.
Template and target information: protein, α-amylase
Author keywords: molecular imprinting, α-amylase, enzyme isolation and purification

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