Abstract: A novel magnetic copper imprinted chitosan/graphene oxide composite biomaterial was prepared by the combination of ion imprinting and inverse suspension crosslinking for selective adsorption of Cu(II) from aqueous solution. High adsorption capacity for copper was obtained with a low level crosslinking and the addition of graphene oxide and triglycine in preparing process. The prepared ion-imprinted magnetic chitosan polymer microsphere (CS-IIP) was characterized by FT-IR, TGA, SEM and EDX. The results indicated that the CS-IIP was prepared successfully and showed good thermo-stability. Effects of different experimental conditions like pH value, contact time and Cu(II) concentration on adsorption capacity were investigated. The adsorption process follows Freundlich isotherm equation and pseudo-second-order kinetic model. The highest adsorption capacity of CS-IIP was 132 mg g-1. The calculation of selective factors and relative selectivity factors of CS-IIP for Cu2+/M2+ (M=Zn, Ni, Co or Cd) was studied. Moreover, the reusability and stability of CS-IIP were investigated too