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Reference type: Journal
Authors: Bai W, Spivak DA
Article Title: A Double-Imprinted Diffraction-Grating Sensor Based on a Virus-Responsive Super-Aptamer Hydrogel Derived from an Impure Extract.
Publication date: 2014
Journal: Angewandte Chemie International Edition
Volume: 53
Issue: (8)
Page numbers: 2095-2098.
DOI: 10.1002/anie.201309462

Abstract: The detection of viruses is of interest for a number of fields including biomedicine, environmental science, and biosecurity. Of particular interest are methods that do not require expensive equipment or trained personnel, especially if the results can be read by the naked eye. A new "double imprinting" method was developed whereby a virus-bioimprinted hydrogel is further micromolded into a diffraction grating sensor by using imprint-lithography techniques to give a "Molecularly Imprinted Polymer Gel Laser Diffraction Sensor" (MIP-GLaDiS). A simple laser transmission apparatus was used to measure diffraction, and the system can read by the naked eye to detect the Apple Stem Pitting Virus (ASPV) at concentrations as low as 10 ng mL-1, thus setting the limit of detection of these hydrogels as low as other antigen-binding methods such as ELISA or fluorescence-tag systems
Template and target information: virus, Apple Stem Pitting Virus, ASPV
Author keywords: Aptamers, molecular imprinting, molecular recognition, Responsive hydrogels, Viruses


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