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Reference type: Journal
Authors: Claude B, Morin P, Denoroy L
Article Title: Selective solid-phase extraction of catecholamines and metanephrines from serum using a new molecularly imprinted polymer.
Publication date: 2014
Journal: Journal of Liquid Chromatography & Related Technologies
Volume: 37
Issue: (18)
Page numbers: 2624-2638.
DOI: 10.1080/10826076.2013.853310

Abstract: A new commercially molecularly imprinted polymer dedicated to the specific solid phase extraction of catecholamines and metanephrines has been tested for the extraction of neurotransmitters in serum samples. A protocol was developed especially for several aminergic neurotransmitters and metabolites (dopamine, 3-methoxytyramine, epinephrine, norepinephrine, normetanephrine, and serotonine). The serum was diluted in water (1/10, v/v), loaded on a cartridge filled with molecularly imprinted polymer and washed successively with water, methanol-water (15/85, v/v), and methanol. Analytes were then eluted with methanol-acetic acid (99/1, v/v). No matrix peak was observed on the chromatograms obtained by high performance liquid chromatography coupled with ultra-violet detection (281 nm). High selectivities were observed between imprinted and nonimprinted polymers. Extraction recoveries ranged from 64-98% (RSD <6%, n = 4) depending on the type of analytes and linearity was assessed in the 0.30-0.90 μM concentration range by least squares regression (r2 > 0.991). Then, large volumes of sample (8-40 mL) were loaded on the cartridge to evaluate the binding capacity of the cartridge. Increasing loaded sample volumes induced a decrease in extraction recovery, particularly for hydrophilic compounds. For a given elution recovery, higher preconcentration factors were achieved (~55) for less hydrophilic compounds (sample volume of 40 mL)
Template and target information: dopamine, 3-methoxytyramine, epinephrine, norepinephrine, normetanephrine, serotonine
Author keywords: catecholamine, metanephrine, molecularly imprinted polymer, preconcentration factor, serum, solid phase extraction


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