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Reference type: Journal
Authors: Berggren C, Bayoudh S, Sherrington D, Ensing K
Article Title: Use of molecularly imprinted solid-phase extraction for the selective clean-up of clenbuterol from calf urine.
Publication date: 2000
Journal: Journal of Chromatography A
Volume: 889
Issue: (1-2)
Page numbers: 105-110.
DOI: 10.1016/S0021-9673(00)00424-6
Alternative URL:

Abstract: A feasibility study was performed in order to study the possibilities in using molecularly imprinted polymers (MIPs) as sorbent material in solid-phase extraction (MISPE) for clean-up of clenbuterol from urine. A binding study of clenbuterol in several solvents was performed on a clenbuterol imprinted polymer as well as on a blank polymer. These binding experiments were used to find suitable loading, washing and elution solvents for the MISPE procedure. Extraction of clenbuterol from calf urine was performed by directly loading a 10-ml urine sample onto the MIP column. Thereafter the column was washed with 10 mi of acetonitrile containing 1% acetic acid, and finally clenbuterol was eluted with 6 mi of methanol containing 10% acetic acid. A recovery of 65% was obtained. This recovery could be increased up to 75% if a sample volume of 1 ml was used or up to 100% if urine was freeze-dried and the residue was dissolved in acetonitrile and spiked with clenbuterol prior to analysis. Chromatograms of the wash and eluate solutions show an efficient clean-up, which supports the potential of MISPE for clean-up of trace amounts of clenbuterol from calf urine. (C) 2000 Elsevier Science BN. All rights reserved
Template and target information: clenbuterol

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