Authors: Kuriu Y
, Kawamura A
, Uragami T
, Miyata T
Formation of Thin Molecularly Imprinted Hydrogel Layers with Lectin Recognition Sites on SPR Sensor Chips by Atom Transfer Radical Polymerization.
Publication date: 2014
Journal: Chemistry Letters
Alternative URL: https://www.jstage.jst.go.jp/article/cl/43/6/43_140103/_article
Abstract: We prepared lectin-imprinted hydrogel layers on surface plasmon resonance (SPR) sensor chips by atom transfer radical polymerization (ATRP). In a buffer solution with a target protein, the SPR signal and binding constant of the lectin-imprinted hydrogel layer chip were much larger than those of the nonimprinted hydrogel layer chip prepared without using a template lectin. By molecular imprinting with ATRP, recognition sites for the target lectin were formed within thin hydrogel layers on the SPR sensor chips. The lectin-imprinted hydrogel layer chips with recognition sites for target protein are candidates for highly sensitive sensor devices for an SPR sensing system
Template and target information: protein, lectin
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