Authors: Senholdt M
, Siemann M
, Mosbach K
, Andersson LI
Determination of cyclosporin A and metabolites total concentration using a molecularly imprinted polymer based radioligand binding assay.
Publication date: 1997
Journal: Analytical Letters
Alternative URL: http://www.informaworld.com/10.1080/00032719708001699
Abstract: Molecular imprinting of cyclosporin A was undertaken and the resultant polymer preparation was applied to the development of a radioligand binding assay for determination of the total concentration of cyclosporin A and its metabolites. The assay method comprised extraction of haemolyzed whole blood with diisopropyl ether followed by competitive radio-'immuno'-assay of the organic phase employing imprinted polymer particles and [H-3]cyclosporin. The assay measured the parent drug and the metabolites AM1, AM9 and AM4N with equal response. Concomitant analysis of patient samples using a specific EMIT assay in parallel with this novel imprinted polymer- based assay permitted simultaneous determination of the concentration of cyclosporin A and that of its metabolites
Template and target information: cyclosporin A
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