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Reference type: Journal
Authors: Ton XA, Acha V, Bonomi P, Tse Sum Bui B, Haupt K
Article Title: A disposable evanescent wave fiber optic sensor coated with a molecularly imprinted polymer as a selective fluorescence probe.
Publication date: 2015
Journal: Biosensors and Bioelectronics
Volume: 64
Page numbers: 359-366.
DOI: 10.1016/j.bios.2014.09.017
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0956566314007027

Abstract: We have developed a disposable evanescent wave fiber optic sensor by coating a molecularly imprinted polymer (MIP) containing a fluorescent signaling group on a 4-cm long polystyrene optical waveguide. The MIP is composed of a naphthalimide-based fluorescent monomer, which shows fluorescence enhancement upon binding with carboxyl-containing molecules. The herbicide 2,4-dichlorophenoxyacetic acid and the mycotoxin citrinin were used as model analytes. The coating of the MIP was either performed ex-situ, by dip-coating the fiber with MIP particles synthesized beforehand, or in-situ by evanescent-wave photopolymerization on the fiber. The sensing element was interrogated with a fiber-coupled spectrofluorimeter. The fiber optic sensor detects targets in the low nM range and exhibits specific and selective recognition over structural analogs and non-related carboxyl-containing molecules. This technology can be extended to other carboxyl-containing analytes, and to a broader spectrum of targets using different fluorescent monomers
Template and target information: 2,4-dichlorophenoxyacetic acid, 2,4-D, citrinin
Author keywords: molecularly imprinted polymer, Signaling monomer, Evanescent wave fiber optic sensor, fluorescence, mycotoxin


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