MIPs logo MIPdatabase      MIP2020 Conference logo Use this space
Custom Search
Reference type: Journal
Authors: Slade CJ
Article Title: Molecular (or bio-) imprinting of bovine serum albumin.
Publication date: 2000
Journal: Journal of Molecular Catalysis B: Enzymatic
Volume: 9
Issue: (1-3)
Page numbers: 97-105.
DOI: 10.1016/S1381-1177(99)00089-2

Abstract: The lyophilization of proteins in the presence of template molecules has been suggested as a means of creating ''de novo'' binding and catalytic sites. The bio-imprinting of bovine serum albumin (BSA) was investigated using the binding of p-hydroxybenzoic acid (pHBA) and the beta-elimination of 4-fluoro-4-(p-nitrophenyl)butan-2-one as model systems. It was found that both binding and catalytic activity could be enhanced by a factor of approximately 3 over that of the native protein but that no specificity was introduced. It was also found that activity was restricted to active groups on the surface of the imprinted proteins which could be influenced by this technique. A synergistic effect between lysine and aspartic acid groups was observed. This suggested that ionised carboxylic acid and amine groups are involved in bio-imprinting and further suggests ways in which the technique could be developed to produce novel protein based catalysts. (C) 2000 Elsevier Science B.V. All rights reserved

  Eat, sleep, imprint, repeat shirt  SMI logo mug  Man of proper-tea mug

Molecules Special Issue call      Appeal for information


Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.

Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:

Mickey Mouse 90th Anniversary banner