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Reference type: Journal
Authors: Zhao KY, Chen T, Lin BB, Cui WK, Kan BH, Yang N, Zhou XY, Zhang XX, Wei JF
Article Title: Adsorption and recognition of protein molecular imprinted calcium alginate/polyacrylamide hydrogel film with good regeneration performance and high toughness.
Publication date: 2015
Journal: Reactive and Functional Polymers
Volume: 87
Page numbers: 7-14.
DOI: 10.1016/j.reactfunctpolym.2014.12.001
Alternative URL: http://www.sciencedirect.com/science/article/pii/S1381514814002508

Abstract: Protein imprinted calcium alginate/polyacrylamide hydrogel film (CA/PAM MIP) with high toughness was prepared using bovine serum albumin (BSA) as template molecule, sodium alginate and acrylamide as functional monomers, N,N'-methylenebisacrylamide (MBAA) as the covalent cross-linker and CaCl2 as the ionic cross-linker via UV radiation-reduced polymerization. Factors affecting the adsorption capacity and imprinting efficiency of the BSA-imprinted CA/PAM hydrogel films were investigated, such as ratio of polyacrylamide/sodium alginate, film thickness, MBAA concentration and CaCl2 concentration. Results showed that the CA/PAM MIP exhibited an obvious improvement in terms of adsorption capacity for BSA compared with non-imprinted polymer (NIP). The adsorption capacity of MIP for BSA reached 22.49 mg/g, which was 2.7 times higher than NIP. The regeneration property of the BSA-imprinted CA/PAM hydrogel was distinctly improved and the imprinting efficiency of CA/PAM MIP maintained 77.95% of the initial value after five repetitions. Single and binary proteins rebinding indicated that the CA/PAM MIP exhibited good recognition performance. Cell culture experiments showed CA/PAM MIP was more suitable for cell culture than CA/PAM NIP. The residual sodium dodecyl sulfate (SDS) in the elution process leaded to the death of mouse fibroblast cells (L929) after 3 days. A moderate elution solution without residue eluent should be used to prepare MIP for cell culture
Template and target information: protein, bovine serum ablbumin, BSA
Author keywords: protein molecular imprinting, Calcium alginate, polyacrylamide, Hydrogel film, adsorption, Recognition, Cell culture

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