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Reference type: Journal
Authors: Yang C, Luan XJ, Liu GF, Zhou XL, Wang J, Hu XY
Article Title: Depletion of Chicken Egg White Proteins by Pending Templates Imprinted Polymers.
Publication date: 2014
Journal: Chinese Journal of Analytical Chemistry
Volume: 42
Issue: (11)
Page numbers: 1651-1655.
DOI: 10.11895/j.issn.0253-3820.140491
Alternative URL: http://210.14.121.5:8080/fxhx/EN/abstract/abstract13713.htm

Abstract: Egg white solutions were used as "pending templates" to prepare molecular imprinted polymers. The obtained polymers acted as stationary phases, exhibiting abilities to deplete abundant proteins in the egg white. The abilities of protein depletion of the imprinted polymers could be adjusted by using different concentrations of egg white in preparing the polymers. After chromatographically processed in a home-made syringe system, abundant proteins such as ovalbumin, lysozyme, and ovotransferrin were removed from egg white sample solutions. Along with the disappearance of the mass spectrum peaks of these proteins, the signals of other components became more obvious. Meanwhile according to reference data and molecular weights, they were reasonably ascribed to be ovalbumin-related proteins, ovotransferrin-related proteins, ovomucoids or flavoproteins respectively. Conclusively, pending imprinting method has the power to deplete abundant proteins, and to retain and enrich posttranslationally modified species at the same time.
Template and target information: pending templates, chicken egg white proteins, protein, ovalbumin, lysozyme, ovotransferrin
Author keywords: Pending templates imprinted polymer, Chicken egg white, protein depletion, enrichment


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