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Reference type: Journal
Authors: Sasaki S, Ooya T, Kitayama Y, Takeuchi T
Article Title: Molecularly imprinted protein recognition thin films constructed by controlled/living radical polymerization.
Publication date: 2015
Journal: Journal of Bioscience and Bioengineering
Volume: 119
Issue: (2)
Page numbers: 200-205.
DOI: 10.1016/j.jbiosc.2014.06.019
Alternative URL: http://www.sciencedirect.com/science/article/pii/S1389172314002527

Abstract: We demonstrated the synthesis of molecularly imprinted polymers (MIPs) with binding affinity toward a target protein, ribonuclease A (RNase) by atom transfer radical polymerization (ATRP) of acrylic acid, acrylamide, and N,N'-methylenebisacrylamide in the presence of RNase. The binding activity of the MIPs was evaluated by surface plasmon resonance (SPR) of the MIP thin layers prepared on the gold-coated sensor chips. The MIPs prepared by ATRP (MIP-ATRP) had a binding affinity toward RNase with larger binding amount compared to MIPs prepared by conventional free radical polymerization methods (MIP-RP). Moreover, protein selectivity was evaluated using reference proteins (cytochrome c, myoglobin, and α-lactalbumin) and was confirmed in MIP-ATRP of optimum film thickness determined experimentally to be 15-30 nm; however, protein selectivity was not achieved in all MIP-RP. We have shown that ATRP is powerful technique for preparing protein recognition materials by molecular imprinting
Template and target information: protein, ribonuclease A, RNase
Author keywords: molecular imprinting, controlled, living radical polymerization, proteins, surface plasmon resonance, molecular recognition, quartz crystal microbalance


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