MIPs logo MIPdatabase      MIP2020 Conference logo Use this space
Custom Search
Reference type: Journal
Authors: Sasaki S, Ooya T, Kitayama Y, Takeuchi T
Article Title: Molecularly imprinted protein recognition thin films constructed by controlled/living radical polymerization.
Publication date: 2015
Journal: Journal of Bioscience and Bioengineering
Volume: 119
Issue: (2)
Page numbers: 200-205.
DOI: 10.1016/j.jbiosc.2014.06.019
Alternative URL: http://www.sciencedirect.com/science/article/pii/S1389172314002527

Abstract: We demonstrated the synthesis of molecularly imprinted polymers (MIPs) with binding affinity toward a target protein, ribonuclease A (RNase) by atom transfer radical polymerization (ATRP) of acrylic acid, acrylamide, and N,N'-methylenebisacrylamide in the presence of RNase. The binding activity of the MIPs was evaluated by surface plasmon resonance (SPR) of the MIP thin layers prepared on the gold-coated sensor chips. The MIPs prepared by ATRP (MIP-ATRP) had a binding affinity toward RNase with larger binding amount compared to MIPs prepared by conventional free radical polymerization methods (MIP-RP). Moreover, protein selectivity was evaluated using reference proteins (cytochrome c, myoglobin, and α-lactalbumin) and was confirmed in MIP-ATRP of optimum film thickness determined experimentally to be 15-30 nm; however, protein selectivity was not achieved in all MIP-RP. We have shown that ATRP is powerful technique for preparing protein recognition materials by molecular imprinting
Template and target information: protein, ribonuclease A, RNase
Author keywords: molecular imprinting, controlled, living radical polymerization, proteins, surface plasmon resonance, molecular recognition, quartz crystal microbalance

  Periodic table Coach hat  Science peptide mug  Gravity waves shirt

Molecules Special Issue call      Appeal for information


Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.

Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:

Mickey Mouse 90th Anniversary banner