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Reference type: Journal
Authors: Wang YZ, Li DY, He XW, Li WY, Zhang YK
Article Title: Epitope imprinted polymer nanoparticles containing fluorescent quantum dots for specific recognition of human serum albumin.
Publication date: 2015
Journal: Microchimica Acta
Volume: 182
Issue: (7-8)
Page numbers: 1465-1472.
DOI: 10.1007/s00604-015-1464-1

Abstract: Epitope imprinted polymer nanoparticles (EI-NPs) were prepared by one-pot polymerization of N-isopropylacrylamide in the presence of CdTe quantum dots and an epitope (consisting of amino acids 598 to 609) of human serum albumin (HSA). The resulting EI-NPs exhibit specific recognition ability and enable direct fluorescence quantification of HSA based on a fluorescence turn-on mode. The polymer was characterized by FT-IR, X-ray photoelectron spectroscopy, transmission electron microscopy and dynamic light scattering. The linear calibration graph was obtained in the range of 0.25-5 μmol mL-1 with the detection limit of 44.3 nmol mL-1. The EI-NPs were successfully applied to the direct fluorometric quantification of HSA in samples of human serum. Overall, this approach provides a promising tool to design functional fluorescent materials with protein recognition capability and specific applications in proteomics
Template and target information: peptide epitope, human serum albumin, protein, HSA
Author keywords: Epitope imprinting, human serum albumin, Quantum dots, Turn-on fluorescence, Fluorometry

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