MIPs logo MIPdatabase     MIP2018 Conference website
Custom Search
Reference type: Journal
Authors: Manickam P, Pasha SK, Bhansali S
Article Title: Antibody-Free Electrochemical Detection of Cortisol Using Molecularly Imprinted Polymer.
Publication date: 2015
Journal: ECS Meeting Abstracts
Volume: MA2015-01
Issue: (40)
Page numbers: 2111.
Alternative URL: http://ma.ecsdl.org/content/MA2015-01/40/2111.abstract

Abstract: Cortisol, an important steroid hormone in the body plays a key role in several stress related diseases including post-traumatic stress disorder [1-2]. Its continuous detection at room temperature still remains a challenge for providing timely health care to patients suffering from such diseases. We report here a novel, highly sensitive, and selective electrochemical cortisol sensor using molecularly imprinted polymer (MIP). MIP are synthetic polymers having highly specific recognition sites selective towards the target analyte and considered to be the versatile, stable and cost-effective alternatives for the natural antibodies. The cortisol specific MIP film was prepared by in situ electropolymerization of pyrrole [3] monomer onto the electrode surface in the presence of cortisol as a template. After removing the cortisol by electrochemical over-oxidation, the specific imprinted sites created on the polymer matrix were used to detect cortisol using ferrocyanide/ferricyanide (K3[Fe(CN)6]/K4[Fe(CN)6]) as a redox mediator. The imprinted sites on the electrode were characterized by cyclic voltammetry and scanning electron microscopy. The effect of experimental variables on the current response such as, electro-polymerization cycles, template/monomer ratio, elution condition for target removal, pH of the supporting electrolyte, and rebinding time were also investigated and optimized. The MIP based cortisol sensor exhibited a good detection limit of 1 pM mL-1cortisol. Moreover, the sensor showed a good reusability, i.e., the sensitivity remained >90% after 6 cycles of elution/rebinding, while the sensitivity only decreased to 90% after 4 weeks of storage at room temperature. -áSimplicity in preparation, high affinity towards cortisol, and stability superior to antibodies makes the MIP technology, a potential candidate for commercializing the cortisol sensor for point-of-care diagnosis. References:[1] Rachel Yehuda, Sarah L Halligan, Robert Grossman, Childhood trauma and risk for PTSD: Relationship to intergenerational effects of trauma, parental PTSD, and cortisol excretion. Development and Psychopathology 13 (2001) 733-753. [2] Syed Khalid Pasha, Ajeet Kaushik, Abhay Vasudev, Shedra Amy Snipes, Shekhar Bhansali, Electrochemical Immunosensing of Saliva Cortisol, Journal of Electrochemial Society 161 (2014) B3077-B3032. [3] Manickam Pandiaraj, Niroj Kumar Sethy, Kalpana Bhargava, Vepa Kameswararao, Chandran Karunakaran, Designing label-free electrochemical immunosensors for cytochrome c using nanocomposites functionalized screen printed electrodes, Biosensors and Bioelectronics 54 (2014) 115-121
Template and target information: cortisol


  Periodic table Stew - the chemical formula for stew shirt  Periodic table Bag bag  Meerkat somebody mention coffee mug

Molecules Special Issue call     Sensors Special Issue call






Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.


Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:
US listings   UK listings

Searching for Molecular Imprinting books on Amazon.com:
Molecular Imprinting
Imprinted Polymers
Polymer Chemistry
Organic Chemistry
Biosensors

Find what you need at Amazon.com

Lab supplies from Amazon