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Reference type: Journal
Authors: Stevenson D
Article Title: Immuno-affinity solid-phase extraction.
Publication date: 2000
Journal: Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences
Volume: 745
Issue: (1)
Page numbers: 39-48.
DOI: 10.1016/S0378-4347(00)00204-8

Abstract: The measurement of trace organics such as drugs and pesticides at low concentration in biological and environmental samples is a challenging analytical task. Despite recent advances in instrumentation most analysts regard sample preparation as the rate-limiting step in the overall analytical method. In recent years there has been a lot of interest in immobilising antibodies onto solid supports such as silica to provide highly selective solid-phase extraction. This paper reviews the use of immune-affinity for solid-phase extraction. It uses as examples extraction of chlortoluron and isoproturon from water and morphine and clenbuterol in urine and plasma respectively. An extensive list of other examples is given. Optimisation procedures are discussed in detail. (C) 2000 Elsevier Science B.V. All rights reserved


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