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Reference type: Journal
Authors: Xu SF, Lu HZ
Article Title: One-pot synthesis of mesoporous structured ratiometric fluorescence molecularly imprinted sensor for highly sensitive detection of melamine from milk samples.
Publication date: 2015
Journal: Biosensors and Bioelectronics
Volume: 73
Page numbers: 160-166.
DOI: 10.1016/j.bios.2015.05.064
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0956566315301627

Abstract: A facile strategy was developed to prepare mesoporous structured ratiometric fluorescence molecularly imprinted sensor for highly sensitive and selective determination of melamine using CdTe QDs as target sensitive dye and hematoporphyrin as reference dyes. One-pot synthesis method was employed because it could simplify the imprinting process and shorten the experimental period. The as-prepared fluorescence MIPs sensor, which combined ratiometric fluorescence technique with mesoporous silica materials into one system, exhibited excellent selectivity and sensitivity. Under optimum conditions, these mesoporous structured ratiometric fluorescence MIP@QDs sensors showed detection limit as low as 38 nM, which was much lower than those non-mesoporous one. The recycling process was sustainable at least 10 times without obvious efficiency decrease. The feasibility of the developed method in real samples was successfully evaluated through the analysis of melamine in raw milk and milk powder samples with satisfactory recoveries of 92-101%. The developed method proposed in this work proved to be a convenient, rapid, reliable and practical way to prepared high sensitive and selective fluorescence sensors with potentially applicable for trace pollutants analysis in complicated samples
Template and target information: melamine
Author keywords: Molecularly imprinted polymers, Ratiometric fluorescence probe, mesoporous silica, Melamine, One-pot synthesis method

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