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Reference type: Journal
Authors: Tang PP, Zhang HP, Huo JC, Lin XY
Article Title: Super-Paramagnetic Nanoparticles by Surface Imprinting on Graphene Oxide Modified Iron (II, III) with Application for the Determination of Ovalbumin by Absorption Spectroscopy.
Publication date: 2015
Journal: Analytical Letters
Volume: 48
Issue: (15)
Page numbers: 2463-2481.
DOI: 10.1080/00032719.2015.1033721

Abstract: Protein surface imprinting produces materials capable of selective recognition and capture of proteins. Herein, a protein surface imprinted polymer on graphene oxide modified super-paramagnetic Fe3O4 nanoparticles is reported. The molecularly imprinted polymer was synthesized by ultrasound-assisted suspension polymerization, using ovalbumin as the template molecule, 3-aminophenylboronie acid as the functional monomer, and methylene-bis-acrylamide as the cross-linking agent. The nanoparticles were approximately 40 nanometers in size and super-paramagnetic. Moreover, these particles demonstrated considerably high adsorption capacity, fast adsorption kinetics, and selective binding affinities toward the template protein ovalbumin. The calibration curve of ovalbumin was linear from 5.0 x 10-11 to 1.0 x 10-10 molar. The limit of detection of ovalbumin was 2.0 x 10-11 M. These results show that this super-paramagnetic material has potential for biological macromolecule separation and determination
Template and target information: protein, ovalbumin
Author keywords: Graphene oxide, Ovalbumin, protein imprinting, Super-paramagnetic nanoparticles


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