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Reference type: Journal
Authors: Surugiu I, Ye L, Yilmaz E, Dzgoev A, Danielsson B, Mosbach K, Haupt K
Article Title: An enzyme-linked molecularly imprinted sorbent assay.
Publication date: 2000
Journal: Analyst
Volume: 125
Issue: (1)
Page numbers: 13-16.
DOI: 10.1039/a908871g
Alternative URL: http://lib3.dss.go.th/fulltext/Journal/analyst/analyst2000/no.1/1,vol.125%202000p13-16.pdf

Abstract: Based on a molecularly imprinted polymer, a competitive binding assay analogous to competitive enzyme immunoassay has been developed. The assay is specific for the herbicide 2,4-dichlorophenoxyacetic acid and uses, for the first time, an enzyme-labelled conjugate as a tracer. The label tobacco peroxidase allowed for colorimetric and chemiluminescence detection. The molecularly imprinted polymer was synthesised in the form of microspheres by precipitation polymerisation. The polymer efficiently and selectively bound the analyte in aqueous solution. Calibration curves were obtained corresponding to analyte concentrations ranging from 40-600 μg mL(- 1) for the colorimetric assay, and from 1-200 μg mL(-1) for chemiluminescence assay
Template and target information: 2,4-dichlorophenoxyacetic acid, 2,4-D

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