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Abstract: A molecularly imprinted polymer (MIP) for melamine was prepared by suspension polymerization, using melamine as a template, methacrylic acid as a functional monomer, and ethylene glycol dimethacrylate as a cross-linker. The optimal template/monomer and monomer/cross-linker ratios were obtained, being defined as 1/15 and 1/12, respectively. Molecular recognition properties and binding capacity toward melamine were evaluated by adsorption tests. Compared with the nonimprinted polymer, the-áimprinted polymer showed higher affinity for melamine with a dissociation constant (Kd) and a maximum binding concentration of 568.4 ± 7.4 μmol/L and 96.8 ± 2.3 μmol/g, respectively. Under optimal conditions, the imprinted polymer was used as a dispersive extraction sorbent to extract melamine from different milk samples, before high performance liquid chromatography with diode array detection (HPLC-DAD) analysis. The validated method presented a limit of detection of 0.6 mg/kg. From the analysis of six different milks, melamine was detected in two of the considered samples at a maximum concentration of 9.3 ± 0.3 mg/kg
Template and target information: melamine
Author keywords: Adsorption High Performance Liquid Chromatography, Matrix effect, molecular imprinting