Reference type: Journal
Authors: Arabi M, Ghaedi M, Ostovan A, Tashkhourian J, Asadallahzadeh H
Article Title: Synthesis and application of molecularly imprinted nanoparticles combined ultrasonic assisted for highly selective solid phase extraction trace amount of celecoxib from human plasma samples using design expert (DXB) software.
Publication date: 2016
Journal: Ultrasonics Sonochemistry
Volume: 33
Page numbers: 67-76.
DOI: 10.1016/j.ultsonch.2016.04.022
Alternative URL: http://www.sciencedirect.com/science/article/pii/S1350417716301201

Abstract: In this work molecular imprinted nanoparticles (MINPs) was synthesized and applied for ultrasonic assisted solid phase extraction of celecoxib (CEL) from human plasma sample following its combination by HPLC-UV. The MINPs were prepared in a non-covalent approach using methacrylic acid as monomer, CEL as template, ethylene glycol dimethacrylate as cross-linker, and 2,2-azobisisobutyronitrile (AIBN) as the initiator of polymerization. pH, volume of rinsing and eluent solvent and amount of sorbent influence on response were investigated using factorial experimental design, while optimum point was achieved and set as 250 mg sorbent, pH 7.0, 1.5 mL washing solvent and 2 mL eluent by analysis of results according to design expert (DX) software. At above specified conditions, CEL in human plasma with complicated matrices with acceptable high recoveries (96%) and RSD% lower than 10% was quantified and estimated. The proposed MISPE-HPLC-UV method has linear responses among peak area and concentrations of CEL in the range of 0.2-2000 μg L-1, with regression coefficient of 0.98. The limit of detection (LOD) and quantification (LOQ) based on three and ten times of the noise of HPLC peaks correspond to blank solution were 0.08 and 0.18 μg L-1, respectively
Template and target information: celecoxib, CEL
Author keywords: Celecoxib, Design expert, HPLC-UV, human plasma, molecular imprinted nanoparticles, solid phase extraction