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Reference type: Journal
Authors: Umpleby RJ, Shimizu KD
Article Title: Toward a standard method for calculating the binding parameters of molecularly imprinted polymers.
Publication date: 2001
Journal: Abstracts of Papers of the American Chemical Society
Volume: 221
Issue: (OLY)
Page numbers: 340.

Abstract: It is proposed that the affinity spectrum (AS) method, as applied to the Langmuir-Freundlich isotherm, be adopted as the standard for calculating the binding parameters of MIPs. The most salient features of this method are that it is generally applicable to MIPs and capable of modeling the heterogeneity found in these materials. Results obtained with the AS method are consistent with those obtained from the popular Scatchard analysis. However, the AS method offers important advantages in that it is completely objective in its approach and does not require extrapolations of the binding isotherm. Finally, one of the greatest advantages of using the AS method is that it allows the facile comparison of MIPs, which is of great importance when investigating variables in the imprinting process or post-synthesis modification of these polymers. Typical parameters of interest, including the total concentration of binding sites, the average association constant, and the quantities of selective and non-selective sites, are also discussed

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