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Reference type: Journal
Authors: Vaidya AA, Lele BS, Kulkarni MG, Mashelkar RA
Article Title: Creating a macromolecular receptor by affinity imprinting.
Publication date: 2001
Journal: Journal of Applied Polymer Science
Volume: 81
Issue: (5)
Page numbers: 1075-1083.
DOI: 10.1002/app.1529

Abstract: A molecularly imprinted polymeric receptor for trypsin was synthesized by employing a novel technique that is a combination of affinity separation and molecular imprinting. An enzyme-inhibitor complex of trypsin and N-acryloyl para- aminobenzamidine was polymerized with acrylamide and N,N'- methylene bis-acrylamide. Template trypsin was extracted out to obtain an affinity-imprinted polymer. Control experiments were performed to demonstrate the synergistic affinity-imprinting effect. The percentage of crosslinker used was the crucial factor in determining the imprinting efficacy of the polymers. Imprinted polymer containing 50% crosslinker exhibited a linear Scatchard plot. Unlike non-imprinted gel, the receptor exhibited almost exclusive recognition of trypsin in an individual batch experiment as well as from a mixture of trypsin and chymotrypsin. (C) 2001 John Wiley & Sons, Inc

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