Reference type: Journal
Authors: Cai QZ, Yang ZY, Chen N, Zhou XM, Hong JL
Article Title: Selective capture and rapid identification of Panax notoginseng metabolites in rat faeces by the integration of magnetic molecularly imprinted polymers and high-performance liquid chromatography coupled with orbitrap mass spectrometry.
Publication date: 2016
Journal: Journal of Chromatography A
Volume: 1455
Page numbers: 65-73.
DOI: 10.1016/j.chroma.2016.05.089
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0021967316307142

Abstract: In the present work, an advanced pretreatment method magnetic molecular imprinted polymers-dispersive solid phase extraction (MMIPs-DSPE) combined with the high sensitivity LTQ-Orbitrap mass spectrometry was applied to the complicated metabolites analysis of Traditional Chinese Medicines (TCMs) in complex matrices. The ginsenoside Rb1 magnetic molecular imprinted polymers (Rb1-MMIPs) were successfully synthesized for specific recognition and selective enrichment of Panax notoginseng saponin metabolites in rat faeces. The polymers were prepared by using Fe3O4@SiO2 as the supporting material, APTES as the functional monomer and TEOS as the cross-linker. The Rb1-MMIPs showed quick separation (10.8 emu/g), large adsorption capacity (636 μmol/g), high selectivity and fast binding kinetics (25 min). Dispersion solid-phase extraction using Rb1-MMIPs (Rb1-MMIPs-DSPE) integrated with LTQ-Orbitrap MS was applied to fish out and identify saponin metabolites from rat faeces, and totally 58 related compounds were detected within 20 min, including 26 PPD-group and 32 PPT-group notoginsenoside metabolites. Parallel tests showed that Rb1-MMIPs-DSPE obtained the lowest matrix effects of 0.98-14.84% and captured the largest number of structural analogues compared with traditional pretreatment methods organic solvent extraction (OSE) and solid phase extraction (SPE)
Template and target information: ginsenoside Rb1, Panax notoginseng saponin metabolites
Author keywords: Magnetic molecularly imprinted polymers, Orbitrap mass spectrometry, Metabolites study, Panax notoginseng, Rat faeces