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Reference type: Journal
Authors: Shumyantseva VV, Bulko TV, Sigolaeva LV, Kuzikov AV, Archakov AI
Article Title: Electrosynthesis and binding properties of molecularly imprinted poly-o-phenylenediamine for selective recognition and direct electrochemical detection of myoglobin.
Publication date: 2016
Journal: Biosensors and Bioelectronics
Volume: 86
Page numbers: 330-336.
DOI: 10.1016/j.bios.2016.05.101
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0956566316305322

Abstract: Electrosynthesis of molecularly imprinted polymer (MIP) templated with myoglobin (Mb) and the reference non-imprinted polymer (NIP) was examined with o-phenylenediamine (o-PD) as a monomer. Mass-sensitive quartz crystal microbalance with dissipation monitoring supplied by an electrochemical module (EQCM-D) was applied to characterize and optimize MIP/NIP electrosynthesis. Mb rebinding was detected by direct electrocatalytic reduction of Mb by square wave voltammetry (SWV) or differential pulse voltammetry (DPV). The results obtained showed high specificity of polymeric antibodies to template Mb, with an imprinting factor determined as a ratio Imax(MIP)/Imax(NIP) of 2-4. The prepared MIP sensor is characterized by an apparent dissociation constant of (3.3 ± 0.5) x 10-9 M and has a broad range of working concentrations of 1 nM-1 μM, with the detection limit of 0.5 nM (9 ng/ml). Mb rebinding was examined in Mb-free diluted human serum spiked with Mb as well as in plasma samples of patients with acute myocardial infarction (AMI) and in control plasma of healthy donors in order to demonstrate the potential medical application of developed MIP sensors
Template and target information: protein, myoglobin, Mb
Author keywords: Molecularly imprinted polymers, myoglobin, Poly-o-phenylenediamine, electropolymerization


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