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Reference type: Journal
Authors: Walshe M, Howarth J, Kelly MT, O'Kennedy R, Smyth MR
Article Title: The preparation of a molecular imprinted polymer to 7-hydroxycoumarin and its use as a solid-phase extraction material.
Publication date: 1997
Journal: Journal of Pharmaceutical and Biomedical Analysis
Volume: 16
Issue: (2)
Page numbers: 319-325.
DOI: 10.1016/S0731-7085(97)00043-5

Abstract: A molecular imprinted polymer (MIP) was prepared to 7-hydroxycoumarin (7-OHC). A number of preparation parameters were examined by ultraviolet (UV) spectroscopy, including the amount of solvent used for reaction, equilibration time, selectivity and capacity of material. The polymer which showed the most selectivity for 7-OHC was then packed into cartridges and used as a solid-phase extraction sorbent. An extraction procedure was then developed from first principles. The cartridges were examined for selectivity of 7-OHC over some other members of the coumarin family. 7-OHC was then extracted from urine using this solid-phase extraction (SPE) method, and its concentration determined using capillary zone electrophoresis (CZE). The method was found to be linear over the range 10-50 mu g ml(-1). Inter-and Intra-assay precision studies were performed ro validate the method. (C) 1997 Elsevier Science B.V

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