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ISBN: 2156-8073
Conference information: 2016 10th International Conference on Sensing Technology (ICST)

Abstract: This paper presents a label-free and non-invasive technique for selective detection of C-terminal telopeptide type I collagen (CTx-I) by employing Electrochemical Impedance Spectroscopy to measure sample impedance. Molecular imprinted polymer, containing artificial recognition sites for CTx-was prepared by precipitation polymerization using CTx-I peptide as a template, methacrylic acid as a functional monomer and ethylene glycol methacrylate as the cross-linker. A high penetration depth planar interdigital sensor was functionalized by a self-assembled monolayer along with the synthesized MIP. Different concentrations of CTx-I sample solutions were tested using the proposed sensing system. High-Performance Liquid chromatography diode array system was used to validate the results
Template and target information: protein, C-terminal telopeptide type I collagen, CTx-I
Author keywords: Bones, coatings, electrodes, Impedance, polymers, scanning electron microscopy, sensors, bone turnover markers, electrochemical impedance spectroscopy, interdigital sensor, molecular imprinted polymers