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Reference type: Journal
Authors: Li L, Peng AH, Lin ZZ, Zhong HP, Chen XM, Huang ZY
Article Title: Biomimetic ELISA detection of malachite green based on molecularly imprinted polymer film.
Publication date: 2017
Journal: Food Chemistry
Volume: 229
Page numbers: 403-408.
DOI: 10.1016/j.foodchem.2017.02.090
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0308814617302881

Abstract: A highly selective and sensitive enzyme-linked immunosorbent assay (ELISA) was developed for the detection of malachite green (MG) using a molecularly imprinted polymer (MIP) film as bionic antibody. The MIP film, based on the self-polymerization of dopamine, was fabricated on the surfaces of a 96-well microplate. It showed specific recognition for MG in aqueous solution. A direct competitive ELISA method was established with the sensitivity reaching 10.31 μg L-1 and the detection limit being 0.3 μg L-1. The cross-reactivity of two structural analogues to MG was less than 10%. The average recovery tested by MG standard spiking was 88.8% for bass and 90.4% for water, and the relative standard deviations were less than 3.6%. All the above results indicated that the developed method could be used to detect MG in fish and water samples rapidly, specifically and accurately
Template and target information: malachite green, MG
Author keywords: Molecularly imprinted polymer film, ELISA, Malachite green, Bionic antibody, Fish, water

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