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Abstract: Molecular imprints (MIPs) composed of CdTe quantum dots and tetracycline-imprinted polymethacrylates were synthesized on the surface of flexible plastic polyimide sheets from methacrylic acid, allyl mercaptan, and ethylene glycol dimethacrylate. The amount of aqueous CdTe solution, ethanol diluent, the reaction time and temperature of the radical-initiated polymerization were optimized. The MIP-QD composites were then copolymerized on the methacrylated polyimides. The resulting films were then immersed into a supersonic water-ethanol bath to strip off the tetracycline (Tc) templates. The fluorescence quenching intensity measured at 565 nm under 315 nm excitation before and after dropping a Tc sample on the film was used to optimize the processes. The calibration plot is linear in the 70 μM to 2.2 mM Tc concentration range at pH 7.5, and the LOD is 8.8 μM. The relative standard deviation is 8.2% (for n = 10). The method was applied to the determination of Tc in spiked samples of bovine serum albumin and fetal bovine serum and gave recoveries of 98% (at 200 μg mL-1) and 97% (at 1.0 ppt), respectively
Template and target information: tetracycline, Tc
Author keywords: Allyl mercaptan, Bovine serum albumin, derivatization, ethylene glycol dimethacrylate, Fetal bovine serum, methacrylic acid, Quenching, polyimide