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Reference type: Journal
Authors: Ye L, Ramström O, Ansell RJ, Mansson MO, Mosbach K
Article Title: Use of molecularly imprinted polymers in a biotransformation process.
Publication date: 1999
Journal: Biotechnology and Bioengineering
Volume: 64
Issue: (6)
Page numbers: 650-655.
DOI: 10.1002/(SICI)1097-0290(19990920)64:6<650::AID-BIT3>3.0.CO;2-7

Abstract: Molecularly imprinted polymers are highly stable and can be sterilised, making them ideal for use in biotransformation process. In this communication, we describe a novel application of molecularly imprinted polymers in an enzymatic reaction. The enzymatic condensation of Z-L-aspartic acid with L-phenylalanine methyl ester to give Z-t-Asp-L-Phe-OMe (Z-aspartame) was chosen as a model system to evaluate the applicability of using molecularly imprinted polymers to facilitate product formation. When the product-imprinted polymer is present, a considerable increase (40%) in product yield is obtained. Besides their use to enhance product yields, as demonstrated here, we suggest that: imprinted polymers may also find use in the continuous removal of toxic compounds during biochemical reactions. (C) 1999 John Wiley & Sons, Inc


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