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Abstract: The molecular imprinting technology is becoming a versatile tool in constructing tailor-made molecular recognition elements. However, no oxidoreductase mimics which can be utilized for the amperometric sensor has ever been developed using molecularly imprinting technology. Glycated hemoglobin, HbA1c, is an important indicator for diabetic control. In order to develop a novel diagnostic system for the measurement of HbA1c, we developed molecularly imprinting technology based artificial enzyme which can catalyze the oxidative cleavage of fructosyl valine, a model compound for HbA1c. Based on our previous report, that polyvinylimidazole functioned as the catalyst for this reaction, we developed the molecularly imprinted catalyst (MIC). Molecularly imprinted catalyst was synthesized by imprinting polyvinylimidazole based polymer using fructosyl valine (Fru-val) that is the model compound for HbA1c as template molecules, based on both typical principles for molecular imprinting, noncovalent and covalent approaches. Fructosyl valine bound to the imprinted polymer more than two times higher than the control polymer, which was prepared in the same way but without the template molecule. The MIC- employing sensor showed high current response to Fru-val compared with the sensor employing the control polymer, due to the increase in the affinity toward cis-diol harboring compound. The amperometric sensor employing MIC showed the selectivity toward Fru-val compared with the sensor signal toward fructosyl-epsilon-lysine (Fru-epsilon-lys), while the sensor employing the control polymer showed almost identical responses to Fru-val as that to Fru-epsilon-lys
Template and target information: fructosyl valine, Fru-val