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Reference type: Journal
Authors: Yilmaz E
Article Title: Orlistat-induced molecular bio-imprinting of microbial lipase.
Publication date: 2003
Journal: World Journal Of Microbiology & Biotechnology
Volume: 19
Issue: (2)
Page numbers: 161-165.
DOI: 10.1023/A:1023216301165

Abstract: Some microbial lipases bio-imprinted with the reversible lipase inhibitor, Orlistat. Comparison of interesterification activities indicated that Orlistat bio-imprinting is equally effective as amphiphile imprinting and yields a 3.5-4.0-fold activity enhancement. Solvent-free incubation medium was as effective as hexane medium. Water addition into the incubation medium erased the bio-imprinting effect; hence, lid opening should be the mechanism of activation. This study provides additional proof to the hypothesis that lipase bio-imprinting is an active-site related phenomenon


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