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Reference type: Journal
Authors: Meng ZH, Yamazaki T, Sode K
Article Title: Enhancement of the catalytic activity of an artificial phosphotriesterase using a molecular imprinting technique.
Publication date: 2003
Journal: Biotechnology Letters
Volume: 25
Issue: (13)
Page numbers: 1075-1080.
DOI: 10.1023/A:1024152229526

Abstract: An artificial phosphotriesterase (PTE) was constructed by co- polymerization of 4(5)-vinylimidazole-Zn2+-methacrylic acid cluster with a divinylbenzene polymer. Compared with the spontaneous hydrolysis, the resulting polymer catalyst caused 10 5-fold rate acceleration towards the hydrolysis of diethyl p- nitrophenyl phosphate ( Paraoxon). The catalytic activity of the polymer catalyst could be enhanced for 30% using molecular imprinting technique and the molecularly-imprinted catalyst (MIC) showed a turnover rate of 7.4 x 10(-2) s(-1) towards the hydrolysis of Paraoxon. The MIC also hydrolyzed thiophosphates and phosphorothiolate triester pesticides. Construction of an amperometric sensor employing the MIC as catalyst achieved a detection limit of 0.1 mM Paraoxon
Template and target information: diethyl p- nitrophenyl phosphate, Paraoxon

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