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Reference type: Journal
Authors: Hennion MC, Pichon V
Article Title: Immuno-based sample preparation for trace analysis.
Publication date: 2003
Journal: Journal of Chromatography A
Volume: 1000
Issue: (1-2)
Page numbers: 29-52.
DOI: 10.1016/S0021-9673(03)00529-6

Abstract: Immuno-based sample preparation techniques are based upon molecular recognition. Thanks to the high affinity and high selectivity of the antigen-antibody interaction, they have been shown to be a unique tool in the sampling area. Immuno-based sample preparation methods include the widely encountered immunoaffinity extraction sorbents, so-called immunosorbents, as well as membrane-based or ultratiltration techniques. This review describes the new developments and applications that have occurred in recent years with emphasis on (i) the antigen- antibody interactions, (ii) and their importance for the properties and use of immunosorbents, (iii) multiresidue extractions, (iv) the on-line coupling to chromatographic or electrophoretic separations, and (v) the high potential for improving MS detection. The recent use of artificial antibodies for sample pretreatment, so-called molecularly imprinted polymers, is also described. (C) 2003 Elsevier Science B.V. All rights reserved

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