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Reference type: Journal
Authors: Theodoridis G, Konsta G, Bagia C
Article Title: Synthesis and evaluation of molecularly imprinted polymers for enalapril and lisinopril, two synthetic peptide anti-hypertensive drugs.
Publication date: 2004
Journal: Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences
Volume: 804
Issue: (1)
Page numbers: 43-51.
DOI: 10.1016/j.jchromb.2003.12.035

Abstract: Molecularly imprinted polymers (MIPs) for the recognition of enalapril and lisinopril were prepared using 4-vinylpyridine as the functional monomer. Following thermal polymerisation the resulting materials were crushed, ground and sieved. First generation MIPs were produced in protic polar porogenic solvents (mixture of methanol (MeOH) and acetonitrile (ACN)). These MIPs were used and validated as sorbents for solid phase extraction and binding assays. Second generation MIPs were produced with polar aprotic porogenic solvent (DMSO). These polymers were packed in HPLC columns in order to investigate their molecular recognition properties in a dynamic mode. The study of the mobile phase composition included two major parameters: organic modifier content and pH value. Retention factors illustrate selective binding of the template from the imprinted polymers, compared to structurally related compounds. (C) 2004 Elsevier B.V. All rights reserved

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