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Reference type: Journal
Authors: Buszewski B, Welerowicz T
Article Title: Stationary phases with special structural properties for high-throughput separation techniques: Preparation, characterization and applications.
Publication date: 2004
Journal: Combinatorial Chemistry & High Throughput Screening
Volume: 7
Issue: (4)
Page numbers: 291-312.

Abstract: Stationary phases with specific structural properties for high- throughput liquid chromatographic (LC) techniques are described. Special attention was paid to phases with special Structural properties, mainly containing internal functional group (e.g. amide). Such materials are generally called "embedded phases". There are phases created in amidation process of aminopropylated silica gel, especially phases based on biological compounds, like phospholipids and cholesterol, which are called immobilized artificial membranes (IAM's). The synthesis and applications of polar embedded amide LC stationary phases were also reviewed. Methods of characterization of synthesized packing materials were presented, with general focusing on spectroscopic measurements like (C-13 and Si-29 CP/MAS NMR and FT-IR), elemental and thermal analysis as well as chromatographic quantitative structure-retention relationships (QSRR) and extended chemometric tests. The potential applications of various dedicated stationary phases in a high-throughput LC screening procedures were also presented


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