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Reference type: Journal
Authors: Zhou HJ, Zhang ZJ, He DY, Hu YF, Huang Y, Chen DL
Article Title: Flow chemiluminescence sensor for determination of clenbuterol based on molecularly imprinted polymer.
Publication date: 2004
Journal: Analytica Chimica Acta
Volume: 523
Issue: (2)
Page numbers: 237-242.
DOI: 10.1016/j.aca.2004.06.068
Alternative URL: http://www.sciencedirect.com/science/article/B6TF4-4D5JVX6-1/2/d95316cf59160550aaf330c059c3115e

Abstract: In this paper, a novel flow chemiluminescence (CL) clenbuterol sensor based on molecularly imprinted polymer (MIP) on line enrichment nanogram clenbuterol and chemiluminescence reaction of potassium permanganate and formaldehyde in the polyphosphate enhanced by clenbuterol. Clenbuterol in the urine was selectively adsorbed on the clenbuterol-imprinted polymer, which was packed into the flow cell. The formaldehyde and the polyphosphate with potassium permanganate flowed through the flow cell and reacted with the on line adsorbed clenbuterol and produced strong CL. The results show that the sensor was reversible. The CL intensity was linear with clenbuterol concentration from 1.0 X 10-9 g/mL to 5.0 X 10-8 g/mL. The detection limit was 3.0 X 10-10 g/mL. The R.S.D. for ng/mL clenbuterol was less than 5% (n = 3). The present method offered a high selectivity and sensitivity that made the quantitative analysis of trace clenbuterol (ng/mL) in the animal urine sample

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