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Reference type: Book Chapter
Authors: Chen YC, Wang ZM, Yan MD, Prahl SA
Publication date: 2004
Chapter title: Steady-state fluorescence anisotropy studies of molecularly imprinted polymer sensors.
Chapter number: 6
Page numbers: 35-39.
DOI: 10.1557/PROC-787-G3.3 Alternative URL: http://www.bme.ogi.edu/~prahl/pubs/pdf/chen03c.pdf

Book title: Molecularly Imprinted Materials-2003
Editors: Kofinas P, Sellergren B, Roberts MJ
Publisher: Materials Research Society
City: Warrendale
Series title: MRS Symposium Proceedings
Volume number: 787

Abstract: Molecularly imprinted polymers (MIPs) are used as recognition elements in biochemical sensors. In a fluorescence-based MIP sensor system, it can be difficult to distinguish the analyte fluorescence from the fluorescence of the polymer itself. We studied steady-state fluorescence anisotropy of anthracene imprinted in a polymer (polyurethane) matrix. Vertically polarized excitation light was incident on MIP films coated on silicon wafers; vertically and horizontally polarized emission was measured. We compared the fluorescence anisotropy of MIPs with imprinted molecules, MIPs with the imprinted molecules extracted, MIPs with rebound molecules, and nonimprinted control polymers. It is shown that differences in fluorescence anisotropy between the polymers and imprinted fluorescent molecules may provide a means to discriminate the fluorescence of analyte from that of the background polymer


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