Authors: Turner NW
, Piletska EV
, Karim K
, Whitcombe M
, Malecha M
, Magan N
, Baggiani C
, Piletsky SA
Effect of the solvent on recognition properties of molecularly imprinted polymer specific for ochratoxin A.
Publication date: 2004
Journal: Biosensors and Bioelectronics
Alternative URL: http://www.sciencedirect.com/science/article/B6TFC-4DJ4C26-1/2/b562785e7d7a91d9fd58b92bc5f16147
Abstract: A molecularly imprinted polymer specific for the mycotoxin ochratoxin A has been synthesised using a non-covalent approach. The polymer has shown an excellent affinity and specificity for the target template in aqueous solutions. The binding experiments, NMR study and molecular modelling have proven that the template recognition by polymer originates from the shape complementarity of binding sites. The binding mechanism is critically depended on factors that affect the polymer conformation. Thus the variation in buffer concentration, pH and presence of organic solvent, which affect the polymer swelling or shrinking, had a profound effect on the polymer recognition properties
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