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Reference type: Journal
Authors: Chen X, Yi CQ, Yang XQ, Wang XR
Article Title: Liquid chromatography of active principles in Sophora flavescens root.
Publication date: 2004
Journal: Journal of Chromatography B
Volume: 812
Issue: (1-2)
Page numbers: 149-163.
DOI: 10.1016/j.jchromb.2004.08.032
Alternative URL: http://www.sciencedirect.com/science/article/B6X0P-4DD937M-2/2/d36c69409c8c43edd126ceabfa73dbdf

Abstract: Herbal medicines were one of the major resources for healthcare in earlier stages, and some traditional herbal medicines have been in use for more than 2000 years. Currently, they are attracting more and more attention of the modern pharmaceutical industry, as scientists has become aware that herbs have almost infinite resources for medicine development. This review provides an overview of the analytical approaches applied in the researches concentrated on various aspects of the matrine-type alkaloids in Sophora flavescens root. Emphasis will be laid on the analytical processes of high-performance liquid chromatography (HPLC), capillary electrophoresis (CE), as well as gas chromatography (GC) methods. The sample extraction, separation and detection have been summarized. In addition, the applications of chromatographic determinations are introduced for the main matrine-type alkaloids in S. flavescens root, such as matrine, sophoridine, sophocarpine, lehmannine, sophoramine, oxymartine, oxysophocarpine, cytosine and aloperine. The advantages and limitations of HPLC, CE and GC methods in the analytical applications of the alkaloids are also discussed

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