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Reference type: Journal
Authors: de Boer T, Mol R, de Zeeuw RA, de Jong GJ, Sherrington DC, Cormack PAG, Ensing K
Article Title: Spherical molecularly imprinted polymer particles: A promising tool for molecular recognition in capillary electrokinetic separations.
Publication date: 2002
Journal: Electrophoresis
Volume: 23
Issue: (9)
Page numbers: 1296-1300.
DOI: 10.1002/1522-2683(200205)23:9<1296::AID-ELPS1296>3.0.CO;2-2

Abstract: Spherical molecularly imprinted polymer particles obtained via precipitation polymerization, were introduced as a pseudostationary phase in capillary electrophoresis (CE) to study molecular recognition. Analyses were performed via a partial filling technique using (+)-ephedrine-imprinted microspheres (100-200 nm) which were polymerized from methacrylic acid and 1,1,1- Tris(hydroxymethyl)propanetrimethacrylate using acetonitrile as the solvent. The influence of pH and the modifier content on the separation was investigated. A 0.1 % w/v suspension in an aqueous 10 mm phosphate buffer (pH 2.5 with 40% acetonitrile) was hydrodynamically injected into the CE system (80% of the effective capillary length) and led to full baseline separation of racemic ephedrine within 10 min


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