Abstract: The molecular imprinting technique is a reliable technique for the preparation of materials of predetermined selectivity. It is highly significant in research of the structure of enzyme, mechanism of receptor-antibody and analytical chemistry. In analytical separation science, molecularly imprinted polymers have been applied in several analytical techniques, such as liquid chromatography, capillary electrochromatography and capillary electrophoresis, solid phase extraction, immuno-assay, and as a selective in chemical sensors. Molecular imprinted polymer for chiral separations have been prepared mainly two approaches: covalent and non-covalent binding of the template and the functional monomer. In this review article, the essential theories, synthesis, manufacture and separation characteristic of molecular imprinting technique are widely described.